Molecular Biology
- Our mission is to carry the
leadership in diagnostic molecular biology for the detection and
monitoring of infectious diseases. To practice and carefully observe
proper handling of all the samples of the patient so as to avoid any
cross contamination and any improper handling of infectious samples. To
ensure that all patients' results are confidential and satisfying.
- Working in our fully operational PCR unit, our highly motivated and
dedicated team uses the latest and most advanced instrumentation and
equipment enabling them to provide quick, accurate and reliable results
in performing stat testing of most aspects of PCR detection.
PURPOSE
- Molecular Biology Department is responsible for the diagnosis of
infectious diseases by using highly sensitive and sophisticated
techniques called Polymerase chain reaction (PCR).
- The advance for effective treatment and care of patient is the rapid
diagnosis of disease and its causative agent at the molecular level
- Due to the ability to amplify small amounts of nuclic acid,PCR can be
used to detect organisms that are difficult to culture in invitro or can
not be cultured.
- Good laboratory techniques are essential for the proper performance of
PCR procedures, to preserve the purity and avoid contamination of the
patient samples for a good quality of results.
- INTRODUCTION about PCR
- From the isolation of specific genes to the sequencing of entire
genomes, the polymerase chain reaction (PCR) has become one of the most
widely used technologies for conducting biological research as well as
diagnostic tool.
- Advances have led to the development of specific and sensitive
high-throughput PCR methods for the detection of a variety of
microorganisms, and these methods are increasingly being applied to
analysis of infectious disease samples.
- The successful application of PCR requires the proper use of
techniques and interpretation of results. The advantages of these
techniques can be offset by the demanding assay protocols and the need
to follow quality assurance/quality control (QA/QC) procedures
carefully. These QA/QC procedures are necessary because the ability of
PCR to produce many copies of target DNA creates the possibility of
contamination by previously amplified products, which can lead to
false-positive results.
- SERVICES
- Department of Molecular Biology provides the following diagnosis of
infectious diseases
- 1. Hepatitis C RNA PCR Qualitative Test
- 2. Hepatitis C RNA PCR Quantitative Test
- 3. Hepatitis B DNA PCR Qualitative Test
- 4. Hepatitis B DNA PCR Quantitative Test
- 5. HIV-1 RNA PCR Test
- Chlamydia trachomatis PCR Test
- Why request HCV RNA Quantitative test?
- a) Measure baseline level of the virus prior to treatment
- b) Monitor therapy once initiated
- c) Predict treatment response
Why request HCV RNA qualitative test?
- a) Differentiate resolved infection from chronic infection
- b) Determine whether a patient has cleared the hepatitis c virus
- c) Monitoring a patient for relapse following therapy
- Why request HBV DNA monitoring test
- a) HBV viral load is useful for determining treatment efficacy
- b) Early detection of potential therapeutic failure
- c) Monitoring HBV in pregnancy
- Why request HIV-1 RNA Monitoring test?
- To achieve maximum sensitivity and dynamic range for the quantitative
detection of HIV-1 RNA in plasma
- Why request Chlamydia trachomatis DNA test?
- a) Chlamydia PCR can be performed on urine sample so eliminates the
need for invasive sampling and simplifying the procedure for obtaining
samples
- b) This test provides a sensitivity approaching
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- The newly formed unit of PCR is in continuation of developing its
services through providing more singular and enhanced detection tests as
a part of the forthcoming operational plans of this component.